A New Radioimmunoassay Method for Serum Triiodothyronine using Sephadex G25 Column
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay of serum triiodothyronine on small, reusable, Sephadex columns.
We have developed a radioimmunoassay method for serum triiodothyronine on small (9 X 23 mm), reusable Sephadex G-25 columns. Recoveries of triiodothyronine added to sera are 97-105%, and the coefficients of variation for within-assay and between-assay determinations are 5.8 and 9.0 % , respectively. Maximum sensitivity is in the hypothyroid range (0100 ng of 13 per dl). Triiodothyronine concent...
متن کاملA two-step radioimmunoassay for free triiodothyronine in serum.
Using a high-affinity solid-phase-bound antibody (Ka = 1.2 X 10(11) L/mol), we have standardized a two-step radioimmunoassay for free triiodothyronine (FT3) in serum, based on immunoextraction. The method was validated by comparison with an equilibrium-dialysis procedure (r = 0.96) involving RIA of T3 in the dialysate standardized with the same antibody and by a commercial (Liso-Phase, Internat...
متن کاملRadioimmunoassay of triiodothyronine in unextracted human serum.
Serum triiodothyronine (T-3) concentrations have been estimated by radioimmunoassay using unextracted serum. The serum T-3 concentrations have been shown to be similar in two separate European populations (0.76-1.67 ng/ml). Raised T-3 values have been observed in all subjects with hyperthyroidism. Low values are seen in hypothyroidism although there is some overlap with the normal range. There ...
متن کاملTriiodothyronine radioimmunoassay.
Highly specific antisera to triiodothyronine (T(3)) were prepared by immunization of rabbits with T(3)-bovine serum albumin conjugates. Antisera with T(3) binding capacity of up to 600 ng/ml were obtained. The ability of various thyronine derivatives to inhibit the binding of T(3-) (125)I to anti-T(3) serum was found to vary considerably. l-T(3), d-T(3) and several triiodoanalogues were potent ...
متن کاملSimplified radioimmunoassay for triiodothyronine: evaluation of a new commercial kit.
A new triiodothyronine (T3) radioimmunoassay kit offers simplicity of method, short incubation time, specificity, and reproducibility. 125I-labeled T3 and unknown serum (or standard) are added to alkaline buffered (pH 12.4) Sephadex G-25 columns; the T3 is freed of proteins with an alkaline buffer wash, antiserum is added, and the columns are incubated at room temperature for 2 h. "Free" hormon...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1973
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.20.365